Product Name

Lipase from Pseudomonas sp.

General deion

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.

Synonym

Triacylglycerol acylhydrolase, Triacylglycerol lipase

Assay

≥15 units/mg solid; Type XIII

CAS Number

9004-02-8

Molecular Weight

mol wt ~134 kDa

MDL number

MFCD00131509

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing Mg₊₂, sodium cholate, and bovine serum albumin as stabilizers

l Biological Information

Biochem/Physiol Actions

Lipase from Pseudomonas was shown to inhibit monocytes chemotaxis in human peripheral blood.

Application

This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37oC by using 2.5 oC~3.0 units of the enzyme per test (3.0ml) at pH around 7.0. Lipase from Pseudomonas sp. has been used in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation.

Unit Definition

One unit will produce 1.0 μmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov