Product Name

Lipase from porcine pancreas

Synonym

PPL, Pancreatic lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase

Assay

Type II, 100-500 units/mg protein (using olive oil (30 min incubation));

30-90 units/mg protein (using triacetin);

Protein(Biuret): ≥20%

CAS Number

9001-62-1

MDL number

MFCD00131509

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized (cake)

l Biological Information

Biochem/Physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester ages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Application

Lipase from porcine pancreas has been used:

• for the analysis of fatty acid at the 2-position of triglycerides.

• to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.

• as a component of dissolution media to study the dissolution profiles of theophylline.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov