Product Name

Lipase from Rhizopus niveus

General deion

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Synonym

Triacylglycerol acylhydrolase, Triacylglycerol lipase

Assay

≥1.5 U/mg

CAS Number

9001-62-1

Molecular Weight

Mr ~83000

MDL number

MFCD00131509

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Fine powder

l Biological Information

Biochem/Physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester age at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate); 300 U as described above are equivalent to ~1 U using triolein at pH 8.0 and 40°C as substrate.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov