Product Name

Cholesterol Esterase from Pseudomonas fluorescens

Synonym

Sterol-ester acylhydrolase

Assay

≥10,000 units/g protein

CAS Number

9026-00-0

MDL number

MFCD00071052

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Tan to Brown lyophilized powder

Solubility(25℃)

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

l Biological Information

Biochem/Physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg₂₊ and 0.3% (v/v) ™ X-100. Ag₊, Hg₂₊ and ionic detergents inhibit the activity of the enzyme.

Application

Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations. This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Preparation Note

Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 (1 mg/mL).  

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov