Product Name

Pectinesterase from orange peel

Synonym

Pectin methylesterase, Pectin pectylhydrolase

Assay

≥150 units/mg protein

CAS Number

9025-98-3

MDL number

MFCD00131812

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Yellow to Light Brown lyophilized powder

Physical form

Contains (NH⁴)²SO⁴ and sodium chloride

l Biological Information

Biochem/Physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Application

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH⁴)²SO⁴ and sodium chloride. It has been used to study the pectin methylesterase gene.

The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Unit Definition

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov