Product Name

Esterase from porcine liver

Synonym

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

Assay

≥150 units/mg protein (biuret)

CAS Number

9016-18-6

MDL number

MFCD00131030

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Brown ammonium sulfate suspension

l Biological Information

Biochem/Physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu. Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Application

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications. Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry. Esterase from porcine liver has been used in a study to assess the reduction of complex coronary atherosclerotic plaque development. Esterase from porcine liver has also been used in a study to investigate the effect of diabetes on bone marrow and circulating porcine endothelial progenitor cells. The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov