Product Name

Esterase from rabbit liver

Synonym

Carboxyl esterase, Carboxylic-ester hydrolase

Assay

≥75 units/mg protein

CAS Number

9016-18-6

MDL number

MFCD00131030

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing Tris buffer salts

l Biological Information

Biochem/Physiol Actions

Esterase is a gylcoprotein having a molecular mass of approximately 60 kDa and is mainly present in the endoplasmic reticulum of hepatocytes. The enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.

Application

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on  and activity of a lipoprotein-associated phospholipase A(2). The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov