Product Name

Ribonuclease A, with scrambled disulfide bonds from bovine pancreas

Assay

PDI substrate; protein ≥50% by UV; ≤2 Kunitz units/mg protein

MDL number

MFCD00132181

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow Powder

Solubility(25°C)

Colorless to Light Yellow to Light Beige ; Clear to Slightly Hazy; 1 mg/mL, 0.01 M HOAc

l Biological Information

Biochem/Physiol Actions

RNA substrates lie in the cleft of RNase A and are cleaved by histidine 12 and histidine 119, via a 2′-3′ cyclic phosphate intermediate. The intermediate is then stabilized by nearby lysines such as Lys7, Lys41 and Lys66.

Application

Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.

Preparation Note

Prepared from Type I-A RNase by the method of Hillson, D.A., et al., Meth. Enzymol., 107, 281 (1984).

l Storage

Storage temp.

−20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov