l General Information |
Product Name | Phosphatase substrate |
Synonym | 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate |
Assay | Contains 40 mg substrate per tablet |
CAS Number | 333338-18-4 | MDL number | MFCD00066288 |
Linear Formula | O2NC6H4OP(O)(ONa)2 · 6H2O | Molecular Weight | 371.14 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Yellow Round Tablet |
Solubility | H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.) |
l Biological Information |
Application | Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm. Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm. The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins. |
Preparation Note | Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
