l General Information |
Product Name | M-MLV Reverse Tranase |
Synonym | Moloney Murine Leukemia Virus Reverse Tranase |
Assay | Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis; 200 units/μL |
CAS Number | 9068-38-6 | MDL number | MFCD00283754 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Colorless solution |
l Biological Information |
Application | M-MLV (Moloney Murine Leukemia Virus) reverse tranase is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. M-MLV is used for the preparation of cDNA libraries or for first strand cDNA synthesis for use in RT-PCR reactions. |
Preparation Note | The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid. |
Unit Definition | One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
