Polynucleotide phosphorylase from Synechocystis sp.
Product #：
S0238
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S0238-100UG
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746.2
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l General Information |
Product Name | Polynucleotide phosphorylase from Synechocystis sp. |
General deion | Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase. |
Synonym | PNPase, Polyribonucleotide Nucleotidyltransferase |
Assay | ≥500 units/mg protein; recombinant, expressed in E. coli |
MDL number | MFCD00131992 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Colorless solution |
l Biological Information |
Biochem/Physiol Actions | Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells. |
Application | Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor. |
Unit Definition | One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C. Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol |
l Storage |
Storage temp. | −70°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
