Product Name

Taq DNA Polymerase from Thermus aquaticus

General deion

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Assay

with 10× PCR reaction buffer without MgCl²

CAS Number

9012-90-2

MDL number

MFCD00166026

Suitability

BioReagent

l Physical and Chemical Information

Appearance

colorless liquid

l Biological Information

Biochem/Physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Application

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping.[1] It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov