Product Name

Myokinase from rabbit muscle

General deion

Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.

Synonym

ATP: AMP phosphotransferase, Adenylate Kinase

Assay

1,500-3,000 units/mg protein (biuret)

CAS Number

9013-02-9

MDL number

MFCD00131644

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White ammonium sulfate suspension

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴ solution containing 0.001 M EDTA, pH 6.0

l Biological Information

Biochem/Physiol Actions

Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.

Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP

Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity.

Application

Myokinase has been used in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate.  It has also been used in a study to investigate the ligand-binding properties of its peptide fragments.

Unit Definition

One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov