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Home > Products > Life sciences > Enzymes and proteins > Creatine Phosphokinase from rabbit muscle

Creatine Phosphokinase from rabbit muscle

Product #: S0226
Image SKU-Pack Size Stock Price($) Quantity
  • S0226-500UN

    In-stock

    117.8

    - +

    S0226-1KU

    In-stock

    142

    - +

    S0226-3.5KU

    In-stock

    144.6

    - +

    S0226-17.5KU

    In-stock

    418.6

    - +

    S0226-35KU

    In-stock

    717.6

    - +

Documents

Details

l General Information

Product Name

Creatine Phosphokinase from rabbit muscle

Synonym

ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase

Assay

≥150 units/mg protein; Type I, salt-free

CAS Number

9001-15-4

MDL number

MFCD00130876  

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Solubility(25)

0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow

l Biological Information

Application

Molecular Weight: ~81,000

Creatine Phosphokinase is a dimer composed predominantly of the skeletal muscle derived homodimer (MM). CK also exists as a heterodimer (MB) particularly in the myocardium. CK derived from brain tissue consists mainly of the brain source homodimer (BB). The amino acid sequences of the M chain and B chains are about 80% homologous. From the sequence, the molecular weight of the M chain is 43,112.

E1%(280)= 8.76

pH Optimum: pH 8.8-9.0 for the forward reaction and pH 6.0-7.0 for the reverse reaction.

CK is a cellular enzyme with a wide tissue distribution. Its physiological role is associated with ATP generation for contractile or transport systems. Increased levels of CK are associated with myocardial infarction, muscular dystrophy, hyperthyroidism, pulmonary infarction and cerebrovascular disease. Variations in relative isozyme distribution can provide additional information in the diagnosis of these conditions. Substrates: Creatine, N-ethylglycocyamine and glyocyamine have been shown to act as substrates for CK. CK is very specific for ATP/ADP. Inhibitors: ADP is a strong inhibitor of the forward reaction competing with ATP. Divalent cations such as Ca2+ (Ki=4.5 mM), Zn2+ and Cu2+ inhibit CK by competing with Mg2+. Other inhibitors include acetate, acetylsalicylic acid, adenosine, p-aminosalicylic acid, AMP, benzoic acid, bicarbonate, bromide, chloride, p-Chloromercuribenzoic acid, ethylene oxide, 2,4-fluorodinitrobenzene, iodide, malonic acid, NAD, nitrate, phosphate, pyrophosphate, salicylic acid, sulfate, sulfite, thyroxine, trichloroacetate, L-triiodothyroxine, L-triiodothyronine, and tripolyphosphate. The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains.

Preparation Note

Produces a clear, colorless to light yellow solution at 5 mg/mL in 0.25 M glycyl-glycine, pH 7.4

Caution

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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