Product Name

Acetate Kinase from Escherichia coli

Synonym

ATP:Acetate phosphotransferase

Assay

≥150 units/mg protein (biuret)

CAS Number

9027-42-3

MDL number

MFCD00130330  

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Physical form

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

l Biological Information

Biochem/Physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.

Involved in the metabolism of propanoate, pyruvate and taurine

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .

Unit Definition

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov