Product Name

Acetate Kinase from Bacillus stearothermophilus

Synonym

ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus

Assay

400-1,200 units/mg solid

CAS Number

9027-42-3

MDL number

MFCD00130330

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Contains Tris-HCl buffer.

l Biological Information

Biochem/Physiol Actions

Involved in the metabolism of propanoate, pyruvate and taurine. Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [₃₂P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [₃₂P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.

Unit Definition

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov