Product Name

Fructose-6-phosphate Kinase, Pyrophosphate-dependent from Propionibacterium freudenreichii (shermanii)

Synonym

6-Phosphofructokinase(pyrophosphate), Pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase

Assay

≥4.0 units/mg protein

CAS Number

55326-40-4

MDL number

MFCD00131108

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Contains imidazole salts and stabilizer

l Biological Information

Biochem/Physiol Actions

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.

Application

FBP was used to study the kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii.

Unit Definition

One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov