Product Name

Polynucleotide Kinase from T4-infected Escherichia coli

Assay

10 units/μL

CAS Number

37211-65-7

Molecular Weight

mol wt 33 kDa

MDL number

MFCD00166009

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Application

Suitable for:

• Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling

• 5′ phosphorylation of oligonucleotides

• Removal of 3′-phosphate groups from phosphorylpolynucleotides

Unit Definition

One unit catalyzes the transfer of one nanomole of 32P to the 5′-end of micrococcal nuclease-treated DNA in 30 min. at 37 °C. Transfer is detected as incorporation into acid-insoluble material.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov