Product Name

Pyruvate Kinase from rabbit muscle

Synonym

ATP:pyruvate 2-O-phosphotransferase, PK

Assay

350-600 units/mg protein; Type VII

CAS Number

9001-59-6

MDL number

MFCD00081946

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 0.01 M phosphate, pH 7.0

l Biological Information

Biochem/Physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Optimal Temperature: 25°C

ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path

Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP. Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Application

Pyruvate kinase from Sigma has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein . The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase.Pyruvate kinase is an enzyme involved in glycolysis and is involved in gluconeogenesis. Product P7768 is from rabbit muscle and is provided in a buffered aqueous glycerol solution and has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency.

Unit Definition

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov