Product Name

Pyruvate Kinase from Bacillus stearothermophilus

Synonym

ATP:pyruvate 2-O-phosphotransferase, PK

Assay

100-300 units/mg protein; Type VIII

CAS Number

9001-59-6

MDL number

MFCD00081946

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White lyophilized powder

Physical form

Lyophilized powder containing Tris buffer salts, pH 8.5

l Biological Information

Biochem/Physiol Actions

Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.

Application

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

Unit Definition

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.2 at 30 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov