Product Name

Pyruvate Kinase from rabbit muscle

General deion

Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.

Synonym

ATP:pyruvate 2-O-phosphotransferase, PK

Assay

350-600 units/mg protein; Type II

CAS Number

9001-59-6

MDL number

MFCD00081946

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White ammonium sulfate suspension

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 6

l Biological Information

Biochem/Physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Optimal Temperature: 25°C

ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path

Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn₂₊).

Application

Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.

Unit Definition

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov