Product Name

Hexokinase from Saccharomyces cerevisiae

Synonym

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

Assay

≥350 units/mg protein, Protein ≥10 % by biuret

CAS Number

9001-51-8

MDL number

MFCD00131321

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing phosphate/citrate pH approx. 7.0

l Biological Information

Biochem/Physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).

D-fructose K_M: 0.33 mM

D-glucose K_M: 0.12 mM

D-mannose K_M: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)

~110 kDa (dimer)

Optimal pH: 7.5 to 9.0

Extinction Coefficient: E_1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg₂₊ions (K_M = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg₂₊ and 4-chloromercuribenzoate)

Preparation Note

Hexokinase is soluble in cold water (0.5–1.0 unit/ml) or citrate buffer, pH 7.0.

Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov