l General Information |
Product Name | Glutathione S-Transferase from human placenta |
General deion | Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. Cytoplasmic isoforms of GST are encoded by five genes, namely α, γ, μ, σ and π. α, μ and π are the most abundant forms in mammals. GSTπ is the isoform of this enzyme found in placenta and is composed of 209 amino acids. |
Synonym | GST, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase |
Assay | 25-125 units/mg protein |
CAS Number | 50812-37-8 | MDL number | MFCD00131198 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
Physical form | Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA. |
l Biological Information |
Biochem/Physiol Actions | Glutathione S-transferase (GST) catalyzes conjugation reactions during phase II metabolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles. Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers. |
Application | Glutathione S-Transferase from human placenta has been used for the immunization of rabbits for the production of specific anti-π-GST antibodies, to determine whether total anti-π-GST antibodies are more specific and reactive as compared to anti-peptide antibodies. |
Preparation Note | Immediately before use, prepare a solution containing 0.075–0.15 unit/ml of Glutathione S-Transferase in 100 mM potassium phosphate buffer, pH 6.5, with 1.0 mM ethylenediaminetetraacetic acid (EDTA). Long term storage of this dilute enzyme solution is not recommended. |
Unit Definition | One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
