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Home > Products > Life sciences > Enzymes and proteins > Glutathione S-Transferase from equine liver

Glutathione S-Transferase from equine liver

Product #: S0197
Image SKU-Pack Size Stock Price($) Quantity
  • S0197-1MG

    In-stock

    87.2

    - +

    S0197-5MG

    In-stock

    256.8

    - +

    S0197-10MG

    In-stock

    442

    - +

    S0197-25MG

    In-stock

    886.6

    - +

Documents

Details

l General Information

Product Name

Glutathione S-Transferase from equine liver

General deion

Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.

Synonym

GST, Glutathione R-transferase, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase

Assay

≥25 units/mg protein

CAS Number

50812-37-8

Molecular Weight

mol wt 45-50 kDa

MDL number

MFCD00131198

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing Tris, reduced glutathione and EDTA.

l Biological Information

Biochem/Physiol Actions

Glutathione S-transferase (GST) from equine liver has been used-

• as a constituent of Tris buffer for incubation of human umbilical vein endothelial cells (HUVEC) with atracurium to assess the proliferation of HUVEC in the presence of atracurium

• as a component of GSB stock solution to determine GSB (glutathione S-bimane) conjugate fluorescence intensity in intact Arabidopsis cells

• as an enzyme standard in spectrophotometric assay to determine the activity of GST

Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

Unit Definition

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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