Product Name

α-2,3-Sialyltransferase from Pasteurella multocida

General deion

Sialyltransferases belongs to the glycosyltransferases family with the capability of catalyzing the transfer of N-acetylneuraminic acid residues. It is a multifunctional enzyme with α-2,3-sialyltransferase activity, α-2,6-sialyltransferase activity, sialidase activity, and trans-sialidase activity. It has a molar mass of 46.4 kDa, with pI, pH being 5.94 and 7.5-8.5 respectively.

Synonym

CMP-N-acetylneuraminate:β-D-galactoside α-(2,3)-N-acetylneuraminyltransferase

Assay

≥2 units/mg protein; recombinant, expressed in E. coli BL21

MDL number

MFCD00130470

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Lyophilized Powder

Physical form

Lyophilized powder containing Tris-HCl and NaCl

l Biological Information

Biochem/Physiol Actions

Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides. Sialyltransferase catalyzes the transfer of Neu5Ac from CMP-Neu5Ac as a donor substrate to the β-D-galactosyl-1,4-N-acetyl-D-glucosaminyl termini of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.

Preparation Note

Reconstitute the lyophilized powder with water to ~5 mg/mL. Solutions can be stored at 2-8°C for 1-2 months after reconstitution. They can also be aliquoted and frozen at -70°C or -20°C for 1year. Multiple freeze-thaw cycles should be avoided.

Unit Definition

One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov