Product Name

α-2,6-Sialyltransferase from Photobacterium damsela

Synonym

β-Galactoside α-2,6-sialyltransferase, CMP-N-Acetylneuraminate:β-D-galactosyl-1,4-N-acetyl-β-D-glucosamine α-2,6-N-acetylneuraminyltransferase

Assay

≥5 units/mg protein; recombinant, expressed in E. coli BL21

MDL number

MFCD00132245

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

l Biological Information

Biochem/Physiol Actions

Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.

Application

Highly active α2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals.

Preparation Note

Reconstitute the lyophilized powder with water to ~5 mg/mL.

Unit Definition

One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.

l Storage

Storage temp.

-20 °C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov