Product Name

Nucleoside Phosphorylase from microorganisms

General deion

Nucleoside phosphorylase is an enzyme important for the biosynthesis of nucleosides.

Synonym

Nucleoside Phosphorylase bacterial, PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

Assay

≥10 units/mg protein

CAS Number

9030-21-1

Molecular Weight

mol wt ~120 kDa

MDL number

MFCD00131739

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing potassium gluconate, mannitol and EDTA

l Biological Information

Biochem/Physiol Actions

Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Application

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation. This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase (XTO-212) and uricase (UAO-201, UAO-211). Purine nucleoside phosophorylase has shown the ability to perform both phosphorylosis and synthesis of purine deoxy- and ribonucleosides.It has also been found that membrane-associated nucleoside phosphorylases may have a transmembranal orientation with their base and ribose-1-P binding sites on opposite sides of the membrane.

Unit Definition

One unit will cause the phosphorolysis of 1.0 μmole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.4 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov