Product Name

Nucleoside Phosphorylase bacterial

Synonym

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

Assay

≥10 units/mg protein; ≥80% protein basis (biuret); recombinant, expressed in E. coli

CAS Number

9030-21-1

MDL number

MFCD00131739

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Lyophilized Powder

l Biological Information

Biochem/Physiol Actions

Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Application

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation. Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov