Product Name

Dextran Sucrase from Leuconostoc mesenteroides

Assay

≥100 units/mg protein

CAS Number

9032-14-8

MDL number

MFCD00130936

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow lyophilized powder

Physical form

Lyophilized powder containing dextran, MES buffer salts and CaCl²

Solubility(25°C)

H²O: soluble 0.9-1.1 mg/mL

l Biological Information

Biochem/Physiol Actions

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer ed mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching ages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.

Application

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.

Unit Definition

One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov