Product Name

Transglutaminase from guinea pig liver

Synonym

Protein-glutamine:amine γ-glutamyltransferase, Protein-Glutamine-γ-Glutamyltransferase

Assay

≥1.5 units/mg protein

CAS Number

80146-85-6

MDL number

MFCD00132446

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Physical form

Lyophilized powder containing Tris and dithioerythritol

Solubility(25℃)

H²O: soluble 1.0 mg/mL, clear

l Biological Information

Biochem/Physiol Actions

Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross ing of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

Application

This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.

Preparation Note

This enzyme is soluble in water (1 mg/ml), yielding a clear solution.

Unit Definition

One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov