Diaphorase from Clostridium kluyveri
Product #:
S0172
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SKU-Pack Size
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Price($)
Quantity
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S0172-100UN
In-stock
167.4
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S0172-300UN
In-stock
413.4
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S0172-500UN
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616.2
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S0172-1.5KU
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1456
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Details
l General Information |
Product Name | Diaphorase from Clostridium kluyveri |
Synonym | Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase |
Assay | 3.0-20.0 units/mg protein (biuret) |
CAS Number | 9001-18-7 | MDL number | MFCD00130947 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Yellow to Yellow-Brown lyophilized powder |
l Biological Information |
Application | Diaphorase from Clostridium kluyveri, or Lipoyl dehydrogenase, has been used in a study to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism. Lipoyl dehydrogenase has also been used in a study to investigate the redox regulation of tyrosine nitration and 3-nitrotyrosine reduction by antioxidants. Methylene blue (MB)-containing polyacrylamide nanoparticle platforms (NPs) were tested in solution with diaphorase from Clostridium kluyveri and the cofactor NADH. this test done done to check whether the encapsulation of MB in NPs could prevent the reduction of MB, and thus protect its photodynamic effectiveness. The enzyme from Sigma has been used along with aldehyde dehydrogenase to construct a biosensor for acetaldehyde by immobilization. It has also been used in the reoxidation of NADP to NADPH. This reaction simultaneously catalyzed the conversion of resazurin into the highly fluorescent resorufin, and also allowed the detection of minute amounts of NAADP. NAADP was initially converted to NADP and NADPH by other enzymes. |
Unit Definition | One unit of either "diaphorase" or "lipoyl" dehydrogenase will oxidize 1.0 μmole of β-NADH per min at pH 7.5 at 25 °C, with the corresponding reduction of 2,6-dichlorophenolindophenol |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |