Product Name

Glutathione Reductase human

Synonym

GR, Glutathione-disulfide reductase, NADPH:oxidized glutathione oxidoreductase

Assay

≥10 units/mg protein, recombinant, expressed in E. coli

CAS Number

9001-48-3

MDL number

MFCD00131196

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Light Yellow to Yellow buffered aqueous solution

Physical form

Solution containing 25 mM Tris-HCl, pH 7.4, 1 mM EDTA, and 50% (v/v) glycerol.

l Biological Information

Biochem/Physiol Actions

Glutathione reductase enzyme is a homodimeric enzyme containing 1 FAD molecule and 1 NADPH binding domain per subunit. Both human GR (hGR) and Plasmodium falciparum GR (PfGR) are essential for the survival of the malaria parasite within the human erythrocyte. Thus, this enzyme may be used for studies of candidate anti-malaria reagents.Glutathione reductase is a ubiquitous flavoenzyme involved in the protection from cell stress. Glutathione reductase catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). It is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also an electron donor for glutathione peroxidases and a substrate for glutathione S-transferases contributing to the detoxification and elimination of toxic electrophilic metabolites and xenobiotics.

Application

Human glutathione reductase has been used in:

• glutathione reductase activity assay

• oxidative stress analysis

• redox assays

Unit Definition

1 unit will reduce 1.0 μmole of DTNB to TNB per minute at 25 °C at pH 7.5.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov