Product Name

DT Diaphorase (NQO1) human

Synonym

DTD, NQO1, Quinone reductase

Assay

≥90% (SDS-PAGE); recombinant, expressed in E. coli

Molecular Weight

monomer mol wt 31000

MDL number

MFCD00130947

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules. DT-diaphorase, also referred to as NAD(P)H:(quinone-acceptor) oxidoreductase, is involved in the reductive activation process of several cytotoxic antitumor quinones and nitrobenzenes. It catalyzes the two-electron reduction of quinones and quinonoid compounds to hydroquinones, using either NADH or NADPH as the electron donor. The flavoenzyme contains one mole of FAD per mole of enzyme.

Application

Human DT diaphorase has been used in a study to assess the development of novel quinone phosphorodiamidate prodrugs. Human DT diaphorase has also been used to investigate its crystal structure for the development of a model for its interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954).

Unit Definition

One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C.

l Packaging & Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov