Product Name

Fructosyl-Amino Acid Oxidase from Corynebacterium sp.

General deion

Enzyme Commission (E.C.) 1.5.3.x; Fructosyl amino acid oxidase [fructosyl-a-l-amino acid:oxygen oxidoreductase] is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide.

Assay

≥0.45 units/mg protein; recombinant, expressed in E. coli

Molecular Weight

mol wt ~88 kDa by electrophoresis

MDL number

MFCD03456889

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Suitable for the determination of fructosyl-L-amino acid. 

Fructosamines are formed when glucose is condensed amino group of amino acids or proteins. Fructosamine oxidases (FAOX) catalyze the oxidative deglycation of low molecular weight fructosamines. Fructosyl amino acid oxidase catalyzes the oxidation of the C-N bond ing the C1 of the fructosyl moiety and the nitrogen of the amino group of fructosyl amino acids.

Application

Fructosyl-amino acid oxidase can be used to detect the levels of glycated proteins, which are markers for diabetes mellitus.

Unit Definition

One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov