Product Name

Diamine Oxidase from porcine kidney

Synonym

Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)

Assay

≥0.05 unit/mg solid

CAS Number

9001-53-0

MDL number

MFCD00130946

Suitability

BioReagent

l Physical and Chemical Information

Appearance

solid

Solubility(25℃)

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

l Biological Information

Biochem/Physiol Actions

Diamine oxidase from porcine kidney is a homodimer consisting of 2 equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.The molecular mass of the enzyme is found to be 170 kDa.The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. It catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. It is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.

Application

Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-ed oligosaccharide structures in diamine oxidase.

Preparation Note

This enzyme is soluble in 100 mM sodium phosphate buffer, pH 7.2 (10 mg/ml).

Unit Definition

One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov