Product Name

L-Amino Acid Oxidase from Crotalus adamanteus

Synonym

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

Assay

≥0.3 unit/mg solid, Type I

CAS Number

9000-89-9

MDL number

MFCD00131454

Suitability

BioReagent

l Physical and Chemical Information

Appearance

dried venom

Solubility(25℃)

H²O: soluble 1.0 mg/mL, clear(lit.)

l Biological Information

Biochem/Physiol Actions

L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver. The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis.

Application

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 °C. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Unit Definition

One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 °C. (L-Leucine is deaminated at the same rate at pH 7.8 at 37 °C.)

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov