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L-Glutamic Dehydrogenase (NADP) from Proteus sp.

Product #: S0145
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Details

l General Information

Product Name

L-Glutamic Dehydrogenase (NADP) from Proteus sp.

Synonym

L-Glutamate:NADP+oxidoreductase (deaminating)

Assay

≥4,000 units/mL

CAS Number

9029-11-2

Molecular Weight

mol wt ~300 kDa

MDL number

MFCD00131461

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide

l Biological Information

Biochem/Physiol Actions

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Application

This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis.

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30 °C in the presence of ammonium ions and NADPH.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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