Product Name

L-Alanine Dehydrogenase from Bacillus subtilis

Synonym

L-Alanine: NAD₊oxidoreductase (deaminating)

Assay

~30 units/mg protein (Lowry)

CAS Number

9029-06-5

MDL number

MFCD00131441

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Clear to Slightly Hazy Colorless to Yellow buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

l Biological Information

Biochem/Physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection.

Unit Definition

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH³ per min at pH 10.0 at 25 °C.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov