Product Name

Pyruvate Oxidase from microorganisms

Synonym

Pyruvate:oxygen oxidoreductase (phosphorylating)

Assay

≥1.5U/mg

CAS Number

9001-96-1

Molecular Weight

mol wt ~260 kD

MDL number

MFCD00132148

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing FAD and sugar as stabilizer

l Biological Information

Biochem/Physiol Actions

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

Application

Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO₂. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.

Unit Definition

One unit will produce 1.0 μmole of H²O² per min during the conversion of pyruvate and phosphate to acetylphosphate and CO² at pH 5.7 at 37 °C.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov