Product Name

Pyruvate Oxidase from Aerococcus sp.

Synonym

Pyruvate: oxygen oxidoreductase (phosphorylating)

Assay

≥35 units/mg protein (biuret)

CAS Number

9001-96-1

MDL number

MFCD00132148

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing buffer salt and sugar

l Biological Information

Biochem/Physiol Actions

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

Application

Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO². It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarum and Streptococcus pneumoniae.

Unit Definition

One unit will produce 1.0 μmole of H²O² per min during the conversion of pyruvate and phosphate to acetylphosphate and CO² at pH 6.7 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov