Product Name

Superoxide Dismutase bovine

General deion

SOD from bovine erythrocytes was the first SOD to be found in mammalian tissues. Before its enzymatic activity was discovered the protein was known as haemocuprein or erythrocuprein. SOD from bovine erythrocytes is a homodimeric non-covalently bound protein with two 16.3 kDa subunits of 151 amino acids. Each monomer has one intrachain disulfide and one free sulfhydryl, one Cu₂₊ atoms and one Zn₂₊ atoms. There are three forms of SOD differentiated by the metal ions in the active site. These are Cu₂₊/Zn₂₊, Mn₂₊, and Fe₂₊ SOD. In vertebrate organisms Cu/Zn-SOD is found in the cytoplasm and the mitochondrial intermembrane space, while Mn-SOD is found in the mitochondrial matrix space. Fe-SOD is found in prokaryotes and some higher plants.

Synonym

CU/ZN-SOD, SOD1, SOD, Superoxide Dismutase 1 bovine, Superoxide: superoxide oxidoreductase

Assay

≥2500 units/mg protein, ≥90% (SDS-PAGE); recombinant, expressed in E. coli

CAS Number

9054-89-1

MDL number

MFCD00132404

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.

Application

Superoxide dismutase has been used in a study to investigate where lipoproteins may affect the L-arginine-nitric oxide pathway. Superoxide dismutase has also been used in a study to investigate the mass spectral evidence for carbonate-anion-radical-induced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase.The product has been used to develop an SOD assay. This assay used dismutase-mediated inhibition of NADH-dependent nitroblue tetrazolium reduction.

Preparation Note

Produced using animal component-free materials.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA⁵⁵⁰ of 0.025 ± 0.005 per min.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov