Product Name

Superoxide Dismutase from bovine liver

General deion

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Synonym

SOD, Superoxide: superoxide oxidoreductase

Assay

2,000-6,000 units/mg protein (biuret)

CAS Number

9054-89-1

MDL number

MFCD00132404

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 3.8 M(NH⁴)²SO⁴, pH 7.0

l Biological Information

Biochem/Physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Application

Superoxide dismutase from bovine liver has been used in a study to assess the inactivation of endothelial derived relaxing factor by oxidized lipoproteins. Superoxide dismutase from bovine liver has also been used in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Preparation Note

SOD is soluble in water (20 mg/ml) yielding a colorless to blue-green solution. SOD is also soluble in aqueous buffers such as 0.1 M potassium phosphate, pH 7.5.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA⁵⁵⁰ of 0.025 ± 0.005 per min.

l Packaging & Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov