Product Name

Cyclooxygenase 1 from sheep

Synonym

COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase

Assay

≥1500 units/mg protein

Molecular Weight

dimer subunit mol wt 70 kDa

MDL number

MFCD02098124

Suitability

BioReagent

l Physical and Chemical Information

Appearance

glycerol solution

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN^® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

l Biological Information

Biochem/Physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H² (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K^M of 8.3 μM for arachidonic acid.

Unit Definition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

l Packaging & Storage

Storage temp.

The enzyme should be stored at –70 °C. After initial defrost, it is recommended the product be aliquoted into tubes and re-frozen at –70 °C to avoid repeated freeze-thaw cycles. Do not store in a frost-free freezer.

COX-1 is relatively unstable at room temperature; to prevent loss of activity during experiments, keep the enzyme on ice (2–8 °C) at all times.

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov