l General Information |
Product Name | Tyrosinase from mushroom |
General deion | Isoelectric point (pI): 4.7-5 pH optimum is 6-7 Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis. Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion. |
Synonym | Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase |
Assay | ≥1000 unit/mg solid |
CAS Number | 9002-10-2 | MDL number | MFCD00082118 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Converts tyrosine to L-DOPA or tyramine to dopamine |
Application | Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population. |
Unit Definition | One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine. |
l Packaging & Storage |
Storage temp. | -20℃ |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
