l General Information |
Product Name | Nitric Oxide Synthase, Inducible from mouse |
General deion | Inducible nitric oxide synthase (iNOS), also known as inflammatory nitric oxide synthase, is a calcium independent isoenzyme, involved in synthesis of nitric oxide (NO). It is a soluble enzyme encoded by the gene mapped to mouse chromosome 11. iNOS is active in dimeric form and its activity is induced by cytokines and various other stimuli. iNOS is expressed in various inflammatory conditions. |
Synonym | Inducible Nitric Oxide Synthase, NOS II, iNOS, macNOS |
Assay | Protein by Bradford: ≥1mg/mL; Enzymatic Activity: ≥4.0 UN/mg; recombinant, expressed in E. coli |
Molecular Weight | mol wt 130 kDa (homodimer) | MDL number | MFCD00803363 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | buffered aqueous solution |
Physical form | Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 8 μM tetrahydrobiopterin |
l Biological Information |
Biochem/Physiol Actions | NOS is responsible for the biosynthesis of nitric oxide from L-arginine. iNOS is not calcium/calmodulin dependent and has a Km = 16 μM for L-arginine. Tumor-derived inducible nitric oxide synthase (iNOS) plays a vital role in stimulating tumor growth and vessel maturation. Therefore, it is considered to be a potential therapeutic target for anti-vascular cancer therapies. Unchecked activity of iNOS leads to overproduction of nitric oxide (NO), which is toxic for living cells. iNOS activity can be controlled at both tranion and translational level by regulating protein stability, dimerization, phosphorylation, cofactor binding and availability of oxygen and L-arginine as substrates. iNOS plays a vital role in excisional wound repair and exhibits gene therapy strategy to advance wound healing process in iNOS-deficient conditions such as diabetes and steroid treatment. |
Application | Nitric Oxide Synthase, Inducible from mouse has been used in immunohistochemical studies. It is also used to evaluate the therapeutic efficacy of inducible nitric oxide synthase (NOS) on reperfusion-induced microcirculatory alterations and hemodynamic adverse effects in the microvasculature of skeletal muscle. |
Unit Definition | One unit will produce 1.0 μmol of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 0.15 mM NADPH, 4.5 μM oxyhemoglobin, 18 μM tetrahydrobiopterin and 180 μM DTT. |
l Packaging & Storage |
Storage temp. | The product should be stored at –70 °C. It will remain active for at least 6 months at that temperature. The enzyme loses ~10% of its activity after a single freeze-thaw cycle. Therefore, after the initial defrost, it is recommended the product be stored in single-use aliquots at –70 °C. During use, keep the solution on ice at all times since the enzyme loses activity at higher temperatures. |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
