Product Name

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

General deion

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe₂₊ as an essential cofactor. Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Synonym

4-HPPD, p-HPPD, a-ketoisocaproate

Assay

recombinant, expressed in E. coli,≥ 1mg/mL(Concentration), ≥ 5UN/mg(Specific Activity)

Suitability

BioReagent

l Biological Information

Unit Definition

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

l Packaging & Storage

Storage temp.

The recommended long-term storage temperature for the product is –70 °C.

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov