Product Name

Lipoxidase from Glycine max (soybean)

Synonym

Linoleate:oxygen oxidoreductase, Lipoxygenase

Assay

≥50,000 units/mg solid

CAS Number

9029-60-1

Molecular Weight

mol wt 108 kDa (two 54 kDa subunits)

MDL number

MFCD00131527

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Contains stabilizer and NaCl

l Biological Information

Biochem/Physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Unit Definition

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov