Product Name

Lipoxidase from Glycine max (soybean)

Synonym

Linoleate:oxygen oxidoreductase, Lipoxygenase

Assay

500,000-1,000,000 units/mg protein

CAS Number

9029-60-1

Molecular Weight

mol wt 108 kDa (two 54 kDa subunits)

MDL number

MFCD00131527

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0

l Biological Information

Biochem/Physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE). Lipoxidase from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

Preparation Note

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov