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Glutathione Peroxidase from bovine erythrocytes

Product #: S0103
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Details

l General Information

Product Name

Glutathione Peroxidase from bovine erythrocytes

Synonym

GSH-Px, Glutathione:hydrogen-peroxide oxido-reductase

Assay

≥300 units/mg protein

CAS Number

9013-66-5

Molecular Weight

mol wt 84.5 kDa

MDL number

MFCD00131195

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts

l Biological Information

Biochem/Physiol Actions

Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione. In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides. Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown.

Application

Glutathione peroxidase from bovine erythrocytes was used as a positive control in cloning and characterization of full-length cDNAs encoding two glutathione peroxidases (GpXs) from Globodera rostochiensis. It was used for the determination of glutathione peroxidase activity in human milk.

Unit Definition

One unit will catalyze the oxidation by H2O2of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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