Phone:| Tech Support:| E-mail:
Home > Products > Life sciences > Enzymes and proteins > Peroxidase from horseradish

Peroxidase from horseradish

Product #: S0101
Image SKU-Pack Size Stock Price($) Quantity
  • S0101-500UN

    In-stock

    168.8

    - +

    S0101-1KU

    In-stock

    245

    - +

    S0101-2KU

    In-stock

    426.4

    - +

    S0101-5KU

    In-stock

    847.6

    - +

Documents

Details

l General Information

Product Name

Peroxidase from horseradish

General deion

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Synonym

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

Assay

Type XII, ≥250 units/mg solid (using pyrogallol); essentially salt-free

CAS Number

9003-99-0

MDL number

MFCD00071339

Suitability

BioReagent

l Physical and Chemical Information

Appearance

essentially salt-free, lyophilized powder

Solubility(25°C)

0.1 M phosphate buffer: soluble (pH 6.0) H2O: soluble

l Biological Information

Biochem/Physiol Actions

HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-ers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase and hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution.When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+,Co2+,Cu2+,Fe3+,Mn2+,Ni2+,and Pb2+ ions.

Application

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8415, type XII, is an essentially salt free lyophilized powder. It is a further purification of product P8375. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used in an aspergillus fumigatus rapid susceptibility assay.The enzyme has been used to determine H2O2 production in tobacco BY-2 cells using a spectrofluorimetric method.

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

天问科技